Title

Zeiss LSM 880 NLO

Location: RRI

Stand: Zeiss AxioObserver - Inverted

Objectives (Mag./NA): 10x/0.3 Air, 20x/0.8 Air, 20x/1.0 Water and 63x/1.4 Oil.

Lamp: HXP fluorescent lamp

Excitation: Single photon 405, 457, 488, 514, 543 and 633 nm lasers. Multi-photon Spectra Physics Mai-Tai DeepSee later tuneable 690-1040 nm.

Emission:

Detectors: Descanned: 2x Multi-alkali PMTs and 1x GaAsP. Non-descanned: 2x Multi-alkali PMTs and 2x GaAsP. Transmitted light detector.

Software: Zen Black

Accessories: Motorised stage and temperature controlled incubation.

 

Used for: In vivo, multi-colour, 3D reconstructions, tiling and co-localisation.

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