Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures.
Manuguerra I., Croce S., El-Sagheer AH., Krissanaprasit A., Brown T., Gothelf KV., Manetto A.
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.