Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Thirty-four lymphoblastoid cell lines that had been previously typed for HLA-DP antigens by primed lymphocyte typing (PLT) were tested by Southern blotting and by ELISA. Using two DP beta probes and a DP alpha probe with a series of enzymes, it is possible to identify restriction fragment length polymorphism (RFLP) patterns characteristic of DPw1, -2, -3, -4, and possibly -5. ELISA typing results, based on two polymorphic DP antibodies DP11.1 and ILR1, were compared with PLT-defined and RFLP-defined types. Thus, using a range of probes and enzymes it is possible to identify DP polymorphism. The value of monoclonal antibodies for such studies is demonstrated, and the molecular data can, in some cases, pinpoint the amino acids responsible for the specificity of the monoclonal antibodies.

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

07/1987

Volume

84

Pages

4596 - 4600

Keywords

Alleles, Antibodies, Monoclonal, Antibody Specificity, Cell Line, DNA, DNA, Recombinant, Enzyme-Linked Immunosorbent Assay, Genetic Markers, HLA-D Antigens, HLA-DP Antigens, Histocompatibility Testing, Humans, Lymphocytes, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length