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To increase the diversity of fluorescent base analogues with improved properties, we here present the straightforward click-chemistry-based synthesis of a novel fluorescent adenine-analogue triazole adenine (A(T)) and its photophysical characterization inside DNA. A(T) shows promising properties compared to the widely used adenine analogue 2-aminopurine. Quantum yields reach >20% and >5% in single- and double-stranded DNA, respectively, and show dependence on neighbouring bases. Moreover, A(T) shows only a minor destabilization of DNA duplexes, comparable to 2-aminopurine, and circular dichroism investigations suggest that A(T) only causes minimal structural perturbations to normal B-DNA. Furthermore, we find that A(T) shows favourable base-pairing properties with thymine and more surprisingly also with normal adenine. In conclusion, A(T) shows strong potential as a new fluorescent adenine analogue for monitoring changes within its microenvironment in DNA.

Original publication

DOI

10.1093/nar/gkr010

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

05/2011

Volume

39

Pages

4513 - 4524

Keywords

Base Pairing, Circular Dichroism, DNA, DNA, Single-Stranded, Deoxyadenosines, Fluorescence, Fluorescent Dyes, Nucleic Acid Denaturation, Organophosphorus Compounds, Spectrometry, Fluorescence, Triazoles