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Hypoxic stress, like DNA damage, induces p53 protein accumulation and p53-dependent apoptosis in oncogenically transformed cells. Unlike DNA damage, hypoxia does not induce p53-dependent cell cycle arrest, suggesting that p53 activity is differentially regulated by these two stresses. Here we report that hypoxia induces p53 protein accumulation, but in contrast to DNA damage, hypoxia fails to induce endogenous downstream p53 effector mRNAs and proteins. Hypoxia does not inhibit the induction of p53 target genes by ionizing radiation, indicating that p53-dependent transactivation requires a DNA damage-inducible signal that is lacking under hypoxic treatment alone. At the molecular level, DNA damage induces the interaction of p53 with the transcriptional activator p300 as well as with the transcriptional corepressor mSin3A. In contrast, hypoxia primarily induces an interaction of p53 with mSin3A, but not with p300. Pretreatment of cells with an inhibitor of histone deacetylases that relieves transcriptional repression resulted in a significant reduction of p53-dependent transrepression and hypoxia-induced apoptosis. These results led us to propose a model in which different cellular pools of p53 can modulate transcriptional activity through interactions with transcriptional coactivators or corepressors. Genotoxic stress induces both kinds of interactions, whereas stresses that lack a DNA damage component as exemplified by hypoxia primarily induce interaction with corepressors. However, inhibition of either type of interaction can result in diminished apoptotic activity.

Original publication

DOI

10.1128/MCB.21.4.1297-1310.2001

Type

Journal article

Journal

Mol Cell Biol

Publication Date

02/2001

Volume

21

Pages

1297 - 1310

Keywords

Acetylation, Apoptosis, Binding Sites, Cell Hypoxia, Cell Line, Cell Nucleus, Cell Transformation, Neoplastic, DNA Damage, Genes, p53, Histone Deacetylase Inhibitors, Histone Deacetylases, Humans, Models, Biological, Nuclear Proteins, Phosphorylation, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-mdm2, RNA, Messenger, Signal Transduction, Transcriptional Activation, Tumor Suppressor Protein p53