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Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits (nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.

Original publication

DOI

10.1002/cphc.201000866

Type

Journal article

Journal

Chemphyschem

Publication Date

25/02/2011

Volume

12

Pages

442 - 461

Keywords

Computational Biology, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Humans, Neoplasms, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Proteins