How Förster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology.
Fruhwirth GO., Fernandes LP., Weitsman G., Patel G., Kelleher M., Lawler K., Brock A., Poland SP., Matthews DR., Kéri G., Barber PR., Vojnovic B., Ameer-Beg SM., Coolen ACC., Fraternali F., Ng T.
Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits (nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.