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The X ray microprobe developed at the Gray Laboratory was originally designed to produce carbon K X rays (278 eV) by electron bombardment and focus them to a few hundred nanometers spot by using a circular diffraction grating with increasing line density (zone plate). The very fine focus achieved (< 0.25 micron radius spot) and the highly localised energy deposition of CK X rays (photoelectron range < 7 nm), represent unique tools to investigate modern radiobiological phenomena. Recent improvements have been directed to increase the dose rate (up to approximately 6 Gy.s-1 entrance dose averaged over a typical V79 cell) and to evaluate the possibility of using higher energy photons (AlK of 1.48 keV). The efficiency of the microprobe system has been tested by assessing the clonogenic potential of V79 cells irradiated with CK X ray beams of different sizes (5 and 0.25 microns radius) and investigating the relevance of the spatial distribution of cells for the bystander effect.

Type

Journal article

Journal

Radiat Prot Dosimetry

Publication Date

2002

Volume

99

Pages

287 - 288

Keywords

Animals, Cell Line, Cell Survival, Cricetinae, Cricetulus, Dose-Response Relationship, Radiation, X-Rays