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We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

Original publication

DOI

10.1117/12.590201

Type

Journal article

Journal

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Publication Date

16/08/2005

Volume

5705

Pages

23 - 31