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Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.

Original publication

DOI

10.1039/c1cc10743g

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

14/06/2011

Volume

47

Pages

6257 - 6259

Keywords

Alkynes, Azides, Click Chemistry, Copper, Cyclization, DNA, Fluorescent Dyes, Oligonucleotides