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Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.

Original publication

DOI

10.1039/c0ob00451k

Type

Journal article

Journal

Org Biomol Chem

Publication Date

07/01/2011

Volume

9

Pages

232 - 235

Keywords

Molecular Structure, Oligodeoxyribonucleotides, Time Factors