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3-Aminophenyl-modified analogues of the bicyclic nucleoside N-methyl-3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one were synthesised and incorporated directly into triplex-forming oligonucleotides in order to utilise their extended hydrogen bonding motif for recognition of the CG base pair. All analogues demonstrated strong binding affinity and very good selectivity for CG from pH 6.2 to 7.0; a marked improvement on previous modifications.

Original publication

DOI

10.1039/c0ob00119h

Type

Journal article

Journal

Org Biomol Chem

Publication Date

21/11/2010

Volume

8

Pages

5087 - 5096

Keywords

Base Pairing, DNA, Fluorescence, Hydrogen-Ion Concentration, Models, Molecular, Nucleic Acid Conformation, Nucleic Acid Denaturation, Nucleosides, Pyrroles, Ultraviolet Rays