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2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases.

Original publication

DOI

10.1016/j.bmc.2010.07.005

Type

Journal article

Journal

Bioorg Med Chem

Publication Date

01/09/2010

Volume

18

Pages

6389 - 6397

Keywords

DNA Footprinting, Humans, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Structure-Activity Relationship, Uridine