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Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.

Original publication

DOI

10.1002/cbic.200800006

Type

Journal article

Journal

Chembiochem

Publication Date

23/05/2008

Volume

9

Pages

1280 - 1285

Keywords

Alkynes, Azides, Circular Dichroism, Cross-Linking Reagents, DNA, Deoxyuridine, Electrophoresis, Capillary, Molecular Structure, Oligonucleotides, Time Factors