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Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

Original publication

DOI

10.1039/c8cc00738a

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

01/05/2018

Volume

54

Pages

4529 - 4532

Keywords

Alkynes, Azides, Click Chemistry, DNA, Green Fluorescent Proteins, Mutation, Nanostructures, Oligodeoxyribonucleotides, Polymerase Chain Reaction, Protein Engineering, Triazoles