Flow Cytometry is a technology enabling the measurement of numerous parameters, via fluoroescence signalling, in your suspension sample of cells/particles.
Lasers in the machine will excite fluorophores, chemical dyes, or fluorescent tags causing them to emit lights at various wavelengths which is directed to a filter array and then to the photomultiplier tube (PMT) detectors. Depending on which fluorescence emitter you decide to employ this allows for the measurement of protein expression, cellular proliferation, and more.
Data can be gathered on a single cell basis at a rate of thousands per second; or a particular population can be sorted, in bulk or single cell, for further experimentation such as cloning, proteomics, or single cell RNA seq.
We aim to provide each user with skilled services, education and training to achieve high quality research.
Our team
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David Malone
Flow Cytometry Specialist
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Rhodri Wilson
Microscopy and Flow Cytometry SRF Lead
Our Facility currently has
2 analysers:
BD FACSCalibur - 2 laser (B/R) with 4 fluoroescene detectors
Attune NXT - 4 lasers (V/B/Y/R) with 14 fluoroescence detectors
and 2 sorters:
BD FACSort - 1 laser (B) with 5 fluoroescence detectors
BD Aria III - 3 lasers (V/B/R) with 11 fluoroescence detectors, allows 4 way sorts and single cell index sorting in plates.
To book a sort with the Aria III please email David
Flow Cytometry Support
Our team is happy to help with any of your flow cytometry based needs
- Training how to use our machines
- Tutoring on flow cytometry theory eg compensation
- Optomising your staining protocols
- Designing complex panels
- Helping with analysis
Panel Design on the Attune or Aria
Our more advanced machines have been registered on FluoroFinder. This online app helps design complex staining panels, can suggest potential fluorophores, and tell you which companies you can get your specific antibodies from.
If you prefer a more manual approach the Attune NxT and Aria III setups are:
Please email David for the excel sheet.