EMI-137 binding affinity and specificity: A, Saturation binding assay showing the Kd value of EMI-137 is 8.72 nmol/L. B, Competitive binding assay of EMI-137 indicating the IC50 value of unlabeled EMI-137 is 25 nmol/L. C, Live-cell microscopy FLO-1 cells showing an absence of immunofluorescence staining for c-MET, whereas there is a strong signal in OE33 cells at 2 hours after the addition of EMI-137. Fluorescence in OE33 cells was markedly reduced when cells were exposed to a mixture of 100 nmol/L unlabeled EMI-137 with 10 nmol/L EMI-137. Scale bar, 50 μm. AU, Arbitary unit.