The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libraries in HeLa cervical cancer cells in 96-well format. We detail reverse transfection of cells with siRNAs, followed by ionizing radiation, fixing, and staining of the plates for automated colony counting. This protocol can be used across a broad range of cell types. For complete details on the use and execution of this protocol, please refer to Tiwana et al. (2015).
Journal article
2022-06-17T00:00:00+00:00
3
Cancer, Cell Biology, Cell culture, Cell-based Assays, High Throughput Screening, Molecular Biology, Single Cell, Gene Library, High-Throughput Screening Assays, RNA, Small Interfering, Radiation, Ionizing, Transfection