Short hairpin RNA (shRNA)-pooled screening is a valuable and cost-effective tool for assaying the contribution of individual genes to cell viability and proliferation on a genomic scale. Here we describe the key considerations for the design and execution of a pooled shRNA screen to identify determinants of radiosensitivity.
Journal article
2016-01-01T00:00:00+00:00
1470
103 - 119
16
Genomic screening, Lentivirus, Pooled library, Radiosensitivity, shRNA, Anti-Bacterial Agents, Cell Count, Gene Library, Genomics, HEK293 Cells, Hexadimethrine Bromide, Humans, Polymerase Chain Reaction, RNA, Small Interfering, Radiation Tolerance