Homologous Recombination and Nonhomologous End-Joining Repair in Yeast
Jones RE., Humphrey TC.
DNA is inherently unstable and is subject to a range of lesions that threaten genome stability. DNA double-strand breaks (DSBs) are the most life threatening of these, which, if unrepaired, can be lethal and also cause chromosomal rearrangements. To prevent such undesirable outcomes, DSBs are repaired by two evolutionarily conserved pathways: homologous recombination (HR) and nonhomologous end-joining (NHEJ). In this chapter, we review the HR and NHEJ repair pathways in yeast, the study of which has provided important paradigms for our understanding of DSB repair in all eukaryotes.