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Metabolic reprogramming is a common hallmark in cancer. The high complexity and heterogeneity in cancer render it challenging for scientists to study cancer metabolism. Despite the recent advances in single-cell metabolomics based on mass spectrometry, the analysis of metabolites is still a destructive process, thus limiting in vivo investigations. Being label-free and nonperturbative, Raman spectroscopy offers intrinsic information for elucidating active biochemical processes at subcellular level. This review summarizes recent applications of Raman-based techniques, including spontaneous Raman spectroscopy and imaging, coherent Raman imaging, and Raman-stable isotope probing, in contribution to the molecular understanding of the complex biological processes in the disease. In addition, this review discusses possible future directions of Raman-based technologies in cancer research.

Original publication

DOI

10.3390/cancers13071718

Type

Journal article

Journal

Cancers (Basel)

Publication Date

05/04/2021

Volume

13

Keywords

Raman imaging, Raman spectroscopy, cancer metabolism, coherent Raman anti-Stokes scattering, lipid metabolism, stable isotope probing, stimulated Raman scattering