Quantitative methods for the analysis of CFTR transcripts/splicing variants.
Amaral MD., Clarke LA., Ramalho AS., Beck S., Broackes-Carter F., Rowntree R., Mouchel N., Williams SH., Harris A., Tzetis M., Steiner B., Sanz J., Gallati S., Nissim-Rafinifa M., Kerem B., Hefferon T., Cutting GR., Goina E., Pagani F.
In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.