Figure 2 from VCAM-1–targeted MRI Improves Detection of the Tumor-brain Interface
Cheng VWT., de Pennington N., Zakaria R., Larkin JR., Serres S., Sarkar M., Kirkman MA., Bristow C., Croal P., Plaha P., Campo L., Chappell MA., Lord S., Jenkinson MD., Middleton MR., Sibson NR.
<p>Microvascular and tumor cell biology at the tumor rim in U87MG glioblastoma. Representative histologic sections of rat brains, intrastriatally injected with U87MG tumor cells, from the tumor core, tumor rim, and the contralateral striatum, with corresponding box and whisker plots of marker expression in each region. Data shown as median ± interquartile range. Sections were immunohistochemically stained (brown) for tumor cell marker vimentin (<b>A</b>), endothelial marker CD31 (<b>B</b>), cell adhesion molecule VCAM-1 (<b>C</b>), cell proliferation marker Ki67 (<b>D</b>), and two stemness markers: SOX2 (<b>E</b>) and nestin (<b>F</b>). Scale bar = 100 μm. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; <i>n</i> = 5; <i>post hoc</i> Bonferroni multiple comparison test for tumor cell density and microvessel density, and <i>post hoc</i> Dunn test for VCAM-1 expression. Tumor core was delineated from the infiltrative border as indicated by the blue and red lines, respectively. Expression of Ki67, nestin, and SOX2 has been normalized to tumor area. **, <i>P</i> < 0.01; matched Wilcoxon test.</p>