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Optical Projection Tomography (OPT) suffers from additive CCD detector noise. This paper investigates the nature of the CCD detector noise for an OPT setup. The CCD detector noise is found to be described well by an intensity dependent zero-mean Gaussian distribution. A method of reducing the effect of noise in fluorescence OPT reconstructions is discussed. The results of the noise reduction are demonstrated using both artificial scenes created with fluorescent microspheres and real auto-fluorescence OPT projections of the cuticle of the amphipod crustacean Parhyale hawaiensis. ©2008 IEEE.

Original publication




Conference paper

Publication Date



56 - 59