Improved in vitro human tumor models for cancer gene therapy.
Jakeman PG., Hills TE., Tedcastle AB., Di Y., Fisher KD., Seymour LW.
Developing effective anticancer treatments is a particular challenge, as agents must contend with not only the target cellular biology, but also with the complex tumor microenvironment. Here we discuss various in vitro strategies that have sought to address this issue, with a particular focus on new methodologies that utilize clinical samples in basic research and their application in gene therapy and virotherapy.