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Enadenotucirev (EnAd) is a group B oncolytic adenovirus developed for systemic delivery and currently undergoing clinical evaluation for advanced cancer therapy. For differentiated carcinomas, systemic delivery would likely expose virus particles to the basolateral surface of cancer cells rather than the apical surface encountered during natural infection. Here, we compare the ability of EnAd and adenovirus type-5 (Ad5) to infect polarised colorectal carcinoma cells from the apical or basolateral surfaces. Whereas Ad5 infection was more efficient via the apical than basolateral surface, EnAd readily infected cells from either surface. Progeny particles from EnAd were released preferentially via the apical surface for all cell lines and routes of infection. These data further support the utility of group B adenoviruses for systemic delivery and suggest that progeny virus are more likely to be released into the tumour rather than back through the basolateral surface into the blood stream.

Original publication

DOI

10.1016/j.virol.2017.02.011

Type

Journal article

Journal

Virology

Publication Date

05/2017

Volume

505

Pages

162 - 171

Keywords

Adenovirus, Cell polarisation, Colorectal cancer, Oncolytic virus, Tight junctions, Tumour infiltration, Adenoviruses, Human, Antineoplastic Agents, Caco-2 Cells, Cell Line, Tumor, Cell Polarity, Colorectal Neoplasms, Epithelial Cells, HT29 Cells, Humans, Microscopy, Electron, Transmission, Oncolytic Virotherapy, Oncolytic Viruses, Receptors, Virus, Tight Junctions, Virus Internalization