Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Adenomatous polyposis coli (APC), a dominantly inherited disorder, has been mapped to chromosome 5q15-q21 by family linkage studies. Cells from patients with deletions in this region, in one case associated with polyposis in a family, have been used to construct human hamster hybrid cell lines that retain either the normal or deleted chromosome 5. These lines have been used to identify markers from the region of the polyposis gene obtained by cloning the ends of 0.5- to 2-megabase BssHII fragments purified by pulsed-field gel electrophoresis. Three markers are described that map within the deletions and must therefore be close to the APC gene.

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

12/1989

Volume

86

Pages

10118 - 10122

Keywords

Adenoma, Adenomatous Polyposis Coli, Base Sequence, Blotting, Southern, Cell Line, Cell Transformation, Viral, Chromosome Deletion, Chromosomes, Human, Pair 5, Cloning, Molecular, Dinucleoside Phosphates, Gene Library, Genes, Dominant, Herpesvirus 4, Human, Humans, Karyotyping, Molecular Sequence Data, Nucleic Acid Amplification Techniques, Oligonucleotide Probes, Restriction Mapping