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Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3rd generation lentivirus is very robust and it typically takes about 1-2 weeks to get stable expression for most mammalian cell lines. The advantage of using the 3rd generation lentivirus are that are very safe and they are replication incompetent.

Original publication

DOI

10.21769/BioProtoc.3073

Type

Journal article

Journal

Bio Protoc

Publication Date

05/11/2018

Volume

8

Keywords

Gene knockdown, Gene over-expression, Lentivirus, Mammalian cell lines, Stable cell lines, Transduction