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In an immunological screening of a mouse testicular cDNA library with a human CREST serum we isolated five overlapping cDNA clones encoding the mouse homolog of a Golgi coatomer complex protein (accession number AF043120), designated beta'-COP in bovine and p102 in humans. We generated antibodies against this protein which specifically recognize the Golgi apparatus of mouse spermatocytes. FISH analyses assigned the beta'-COP gene Copb2 to mouse Chromosome 9, region E3-F1. Our results demonstrate that CREST sera can contain antibody components against Golgi proteins as well as against nuclear proteins.

Original publication

DOI

10.1159/000015467

Type

Journal article

Journal

Cytogenet Cell Genet

Publication Date

1999

Volume

87

Pages

201 - 204

Keywords

Animals, Antibody Specificity, Autoantigens, Blotting, Western, CREST Syndrome, Cloning, Molecular, Coatomer Protein, Gene Library, Golgi Apparatus, Humans, Immune Sera, In Situ Hybridization, Fluorescence, Male, Mice, Molecular Sequence Data, Physical Chromosome Mapping, Sequence Homology, Amino Acid, Spermatocytes