Different patterns of G/T and A/C mismatch repair in simian cells correspond to the specificity of a mismatch binding protein isolated from simian and HeLa cells.
Brown TC., Corman N., Rudkin BB., Jiricny J.
The two transition mispairs G/T and A/C are corrected with different efficiencies and specificities in CV-1 African green monkey kidney cells. G/T mispairs are corrected with 99% efficiency and almost always in favor of guanine, while A/C mispairs exhibit a 31% uncorrected sector and are otherwise randomly corrected. The higher correction efficiency and bias for the G/T mispair can be correlated with the substrate specificity of a protein, found in HeLa and CV-1 cell extracts, that binds selectively to oligonucleotide duplexes containing G/T mispairs. No binding can be detected to duplexes containing other mispairs, or to homoduplexes.