A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants.
Kennedy PG., Watkins BA., LaThangue NB., Clements GB., Thomas DG.
Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV-specified proteins, and one monoclonal antibody T181 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. T181 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the T181-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV-1 infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.