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The critical pathways determining the resistance of tumor cells to ionizing radiation are poorly defined. Because the ras oncogene, a gene activated in many human cancers treated with radiotherapy, can induce increased radioresistance, we have asked which Ras effector pathways are significant in conferring a survival advantage to tumor cells. The phosphoinositide-3-kinase (PI3K) inhibitor LY294002 radiosensitized cells bearing mutant ras oncogenes, but the survival of cells with wild-type ras was not affected. Inhibition of the PI3K downstream target p70S6K by rapamycin, the Raf-MEK-MAPK pathway with PD98059, or the Ras-MEK kinase-p38 pathway with SB203580 had no effect on radiation survival in cells with oncogenic ras. Expression of active PI3K in cells with wild-type ras resulted in increased radiation resistance that could be inhibited by LY294002. These experiments have indicated the importance of PI3K in mediating enhanced radioresistance and have implicated PI3K as a potential target for specific radiosensitization of tumors.

Type

Journal article

Journal

Cancer Res

Publication Date

15/05/2001

Volume

61

Pages

4278 - 4282

Keywords

Animals, Chromones, Enzyme Induction, Enzyme Inhibitors, Flavonoids, Genes, ras, Humans, MAP Kinase Signaling System, Morpholines, Phosphatidylinositol 3-Kinases, Radiation Tolerance, Rats, Tumor Cells, Cultured, ras Proteins