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The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.

Original publication

DOI

10.1016/j.jcf.2004.05.004

Type

Journal article

Journal

J Cyst Fibros

Publication Date

08/2004

Volume

3 Suppl 2

Pages

11 - 15

Keywords

Cystic Fibrosis Transmembrane Conductance Regulator, DNA, Complementary, Gene Expression Profiling, Genetic Techniques, Humans, RNA, Reverse Transcriptase Polymerase Chain Reaction