Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Transfection of primary cells with mutated oncogenic ras plus a cooperating oncogene such as myc results in the acquisition of the transformed cell phenotype. The pathways downstream of Ras that are required for transformation are an active topic of research. The Raf-MEKK-MAP kinase pathway is triggered by activation of Ras and thought to be important in Ras transformation of rodent fibroblasts. To further explore the involvement of this pathway, fibroblasts from homozygous knock out c-Raf-1 mouse embryos (20 KO) and wild-type c-Raf-1 mouse embryos (16 WT) were transfected with H-ras and myc(v). The resulting cell line derived from the knock out cells grew slower both in tissue culture and had a longer latency period as tumors than the transformed cell line from the wild-type cells. Both cell lines were however able to form tumors in nude mice. These results suggest that c-Raf-1 is not required for Ras transformation in this system.

Type

Journal article

Journal

Cancer Biol Ther

Publication Date

01/2003

Volume

2

Pages

73 - 75

Keywords

Animals, Blotting, Southern, Blotting, Western, Cell Division, Cell Transformation, Neoplastic, Embryo, Mammalian, Fibroblasts, Gene Expression Regulation, Neoplastic, Genes, myc, Homozygote, Humans, MAP Kinase Signaling System, Mice, Mice, Knockout, Mice, Nude, Proto-Oncogene Proteins c-raf, Proto-Oncogene Proteins p21(ras), Signal Transduction, Transfection, Tumor Cells, Cultured