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Two splice variants of Nek2 kinase, a member of the NIMA-related family, have been identified as Nek2A and Nek2B. Nek2A regulates centrosome disjunction, spindle formation checkpoint signaling, and faithful chromosome segregation. A specific role for Nek2B has not yet been identified. Here, we have examined the distinct roles of Nek2A and Nek2B using timelapse video microscopy to follow the fate of cells progressing through the cell cycle in the absence of either Nek2A or Nek2B. We show that the down-regulation of Nek2B leads to a mitotic delay in the majority of cells. Upon exiting mitosis, cells exhibit mitotic defects such as the formation of multinucleated cells. Such phenotypes are not observed in cells that exit mitosis in the absence of Nek2A. These observations suggest that Nek2B may be required for the execution of mitotic exit.

Original publication

DOI

10.1016/j.bbamcr.2005.01.007

Type

Journal article

Journal

Biochim Biophys Acta

Publication Date

30/06/2005

Volume

1744

Pages

89 - 92

Keywords

Alternative Splicing, Cell Cycle, Cell Cycle Proteins, Centrosome, Down-Regulation, Gene Expression Regulation, Enzymologic, Green Fluorescent Proteins, HeLa Cells, Humans, Image Processing, Computer-Assisted, Isoenzymes, Microscopy, Video, Mitosis, Molecular Weight, NIMA-Related Kinases, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Time Factors