Figure 2 from Targeting c-MET for Endoscopic Detection of Dysplastic Lesions within Barrett’s Esophagus Using EMI-137 Fluorescence Imaging
Huang Y-J., Rieder J., Tan KV., Tenditnaya A., Vojnovic B., Gorpas D., Quante M., Vallis KA.
<p>EMI-137 binding affinity and specificity: <b>A</b>, Saturation binding assay showing the <i>K<sub>d</sub></i> value of EMI-137 is 8.72 nmol/L. <b>B,</b> Competitive binding assay of EMI-137 indicating the IC<sub>50</sub> value of unlabeled EMI-137 is 25 nmol/L. <b>C,</b> Live-cell microscopy FLO-1 cells showing an absence of immunofluorescence staining for c-MET, whereas there is a strong signal in OE33 cells at 2 hours after the addition of EMI-137. Fluorescence in OE33 cells was markedly reduced when cells were exposed to a mixture of 100 nmol/L unlabeled EMI-137 with 10 nmol/L EMI-137. Scale bar, 50 μm. AU, Arbitary unit.</p>