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The intense demand for fluorescence-based point of care (POC) DNA diagnostics is driving developments to reduce the size of instrumentation, imposing limitations on the optical hardware that can be included. Here we describe a combination of instrumentation and fluorogenic probes to detect three fluorophores using two excitation and two detection channels.

Original publication

DOI

10.1039/c3an00593c

Type

Journal article

Journal

Analyst

Publication Date

07/07/2013

Volume

138

Pages

3626 - 3628

Keywords

Base Sequence, DNA, Fluorescent Dyes, Nucleic Acid Denaturation, Point-of-Care Systems, Spectrometry, Fluorescence, Transition Temperature