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Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.

Type

Journal article

Journal

Artif DNA PNA XNA

Publication Date

07/2011

Volume

2

Pages

79 - 89

Keywords

HyBeacon, PNA, SNPs, fluorescence melting, genetic analysis, statin-induced myopathy, Click Chemistry, DNA Mutational Analysis, Fluorescent Dyes, Genotyping Techniques, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Mass Spectrometry, Muscular Diseases, Oligonucleotide Probes, Peptide Nucleic Acids, Polymorphism, Single Nucleotide, Transition Temperature