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Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, E(m)) directly correlates with the calculated nearest neighbour and experimental melting temperatures; T(m), for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.

Original publication

DOI

10.1016/j.bioelechem.2011.11.003

Type

Journal article

Journal

Bioelectrochemistry

Publication Date

06/2012

Volume

85

Pages

7 - 13

Keywords

Base Pairing, Base Sequence, DNA, DNA Probes, Drug Design, Electrochemical Techniques, Nucleic Acid Conformation, Nucleic Acid Denaturation