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A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

Original publication




Journal article


Bioorg Chem

Publication Date





215 - 219


14-3-3 Proteins, Animals, Aptamers, Nucleotide, Base Sequence, Chromatography, Affinity, Cloning, Molecular, DNA, DNA, Single-Stranded, Humans, Molecular Sequence Data, Oligonucleotides, Protein Binding, Recombinant Proteins, SELEX Aptamer Technique, Scrapie, Sheep