Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

Original publication

DOI

10.1016/j.bioorg.2007.12.005

Type

Journal article

Journal

Bioorg Chem

Publication Date

10/2008

Volume

36

Pages

215 - 219

Keywords

14-3-3 Proteins, Animals, Aptamers, Nucleotide, Base Sequence, Chromatography, Affinity, Cloning, Molecular, DNA, DNA, Single-Stranded, Humans, Molecular Sequence Data, Oligonucleotides, Protein Binding, Recombinant Proteins, SELEX Aptamer Technique, Scrapie, Sheep