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The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.

Original publication




Journal article


J Am Chem Soc

Publication Date





6859 - 6864


Alkynes, Azides, Chromatography, High Pressure Liquid, Copper, DNA, Circular, Electrophoresis, Polyacrylamide Gel, Nucleic Acid Denaturation, Oligodeoxyribonucleotides