Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with single A residues were less stable by 8 degrees C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.

Original publication

DOI

10.1016/j.febslet.2007.03.040

Type

Journal article

Journal

FEBS Lett

Publication Date

17/04/2007

Volume

581

Pages

1657 - 1660

Keywords

Base Sequence, DNA, Deoxyribose, G-Quadruplexes, Nucleic Acid Conformation, Oligonucleotides, Thermodynamics