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Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored.

Original publication

DOI

10.1017/S0033583506004197

Type

Journal article

Journal

Q Rev Biophys

Publication Date

11/2005

Volume

38

Pages

311 - 320

Keywords

Base Pairing, Base Sequence, Biophysical Phenomena, Biophysics, DNA, Kinetics, Models, Molecular, Molecular Structure, Nucleic Acid Conformation, Nucleic Acids, Nucleosides, RNA