Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA. While the parent compound (thiazotropsin A) binds to the hexanucleotide sequence ACTAGT, changing one of the N-methylpyrrole groups to N-methylimidazole changes the preferred binding sequence to (A/T)CGCG(T/A). Experiments with DNA fragments that contain variants of this sequence suggest that the ligand can also bind, with lower affinity, to sequences which differ from this by 1bp in any position.

Original publication

DOI

10.1016/j.bmcl.2006.04.007

Type

Journal article

Journal

Bioorg Med Chem Lett

Publication Date

01/07/2006

Volume

16

Pages

3469 - 3474

Keywords

Base Sequence, Binding Sites, DNA, DNA Footprinting, Imidazoles, Ligands, Molecular Sequence Data, Molecular Structure, Nylons, Propane, Spectrometry, Fluorescence, Temperature, Thiazoles