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We examined the stability and kinetics of folding of the Oxytricha telomeric repeat sequence (G4T4)4. Fluorescence melting experiments show that this intramolecular quadruplex, which is more stable in potassium- than sodium-containing buffers, shows considerable hysteresis between the melting and annealing profiles, even when heated at a rate of 0.05 degrees C min(-1). Quantitative analysis of this hysteresis, together with temperature-jump relaxation experiments show that the dissociation is exceptionally slow with a half-life of about 10 years at 37 degrees C in the presence of 50 mM K+. The association reaction has a half-life of a few seconds at 37 degrees C, but becomes slower at elevated temperatures consistent with the suggestion that association occurs by a nucleation-zipper mechanism.

Original publication

DOI

10.1039/b511706b

Type

Journal article

Journal

Org Biomol Chem

Publication Date

21/11/2005

Volume

3

Pages

4153 - 4157

Keywords

Animals, DNA, G-Quadruplexes, Kinetics, Nucleic Acid Conformation, Nucleic Acid Denaturation, Oxytricha, Spectrometry, Fluorescence, Telomere, Temperature, Thermodynamics