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A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

21/04/2003

Pages

970 - 971

Keywords

Anthraquinones, Carbocyanines, Fluorescence Polarization, Genetic Techniques, Oligonucleotide Probes, Oligonucleotides, Spectrometry, Fluorescence