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We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold.


Journal article


Biochim Biophys Acta

Publication Date





137 - 145


Animals, Cattle, DNA, DNA Footprinting, Deoxyribonuclease I, Ligands, Nucleic Acid Conformation, Quinolines