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Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and cytosine PNA monomers were used in conjunction with standard DNA synthesis monomers to produce chimeric PNA DNA (PDC) oligomers. Ultraviolet melting studies confirmed that these oligomers form stable hybrids with complementary DNA strands and that mismatches in the DNA but more so in the PNA sections lead to duplex destabilisation.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

01/09/1996

Volume

24

Pages

3357 - 3363

Keywords

Chemistry, Organic, Nucleic Acid Denaturation, Oligodeoxyribonucleotides, Peptides, Propanolamines, Propylene Glycols