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DNP-labelled phosphoramidites have been used to synthesis oligonucleotides with multiple DNP reporter groups. The antibody-mediated detection and the stability of duplexes formed by these labelled oligonucleotides have been studied. A DNP-labelled deoxyuridine triphosphate has also been used to enzymatically incorporate DNP-labels into DNA via the polymerase chain reaction. The use of DNP-labelled primers in the PCR has also been investigated.

Type

Journal article

Journal

Biomed Pept Proteins Nucleic Acids

Publication Date

1996

Volume

2

Pages

7 - 12

Keywords

Antibodies, DNA Primers, Dinitrobenzenes, Nucleic Acid Hybridization, Oligonucleotides, Polymerase Chain Reaction, Ultraviolet Rays