Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

11/07/1992

Volume

20

Pages

3411 - 3417

Keywords

Base Sequence, Cholesterol, Chromatography, High Pressure Liquid, HIV Core Protein p24, HIV-1, Molecular Sequence Data, Oligonucleotides, Antisense, Temperature, Thionucleotides, Ultraviolet Rays