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A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.


Journal article


Nucleic Acids Res

Publication Date





3411 - 3417


Base Sequence, Cholesterol, Chromatography, High Pressure Liquid, HIV Core Protein p24, HIV-1, Molecular Sequence Data, Oligonucleotides, Antisense, Temperature, Thionucleotides, Ultraviolet Rays