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The synthesis of non-nucleoside-based phosphoramidites that bear the 2,4-dinitrophenyl group is reported. These labelled phosphoramidites, which have been used in solid-phase oligonucleotide synthesis to attach single and multiple dinitrophenyl groups to the 5'-end of oligonucleotides, are entirely compatible with the normal oligonucleotide synthesis cycle. Multiple labelling can be performed readily in high yield and the resulting oligonucleotides can be purified readily by reversed-phase h.p.l.c. The labelled oligonucleotides have been detected using monoclonal and polyclonal anti-dinitrophenyl antibodies.

Type

Journal article

Journal

Carbohydr Res

Publication Date

02/09/1991

Volume

216

Pages

315 - 322

Keywords

2,4-Dinitrophenol, Base Sequence, DNA, Dinitrophenols, Molecular Sequence Data, Oligodeoxyribonucleotides, Polydeoxyribonucleotides